Fig. 2

P2Y12 shRNA relieved the mechanical allodynia, visceral hyperalgesia and depression-like behaviors in IBD mice. a Experimental flowchart. b Time course of mechanical threshold with P2Y12 shRNA treatment. c Visceral hyperalgesia was evaluated by CRD. d After P2Y12 shRNA treatment, mice’s preference for sucrose was recorded as: sucrose consumption(g) / (sucrose consumption(g) + water consumption(g)) × 100%. e Representative results of the effect of the P2Y12 shRNA treatment on the proportion of floating time in the FST. The proportion of floating time was recorded as: (Floating time(s)/total time(s)) × 100%. f Representative results of the effect of the P2Y12 shRNA treatment on the proportion of immobility time in the TST. The proportion of immobility time was recorded as: (Immobility time(s)/total time(g)) × 100%. The data are expressed as mean ± SEM (n = 6–9 mice). *p < 0.05, compared with the control + vectors group; #p < 0.05, compared with the TNBS + vectors group