Fig. 9

EA inhibited the activation of microglia in the mPFC of IBD mice. a Representative images showing CD68 immunoreactivity in the mPFC of IBD mice. Represented microglial activated stained red with CD68. Scale bars: 200 μm. b Magnified representative images showing CD68 immunoreactivity in the mPFC of IBD mice. Represented microglial activated stained red with CD68. Scale bars: 50 μm. c CD68 are stained red and nuclei are stained blue with DAPI in the mPFC of IBD mice. Scale bars: 200 μm. d CD68 are stained red and nuclei are stained blue with DAPI in the mPFC of IBD mice. Scale bars: 50 μm. e The percentage of CD68-positive area. Data are presented as the mean ± SEM (n = 4). *p < 0.05, compared with the control group; ^#p < 0.05, compared with the TNBS group; ^Δp < 0.05, compared with the EA group