Fig. 7

The main monomers of PAC and AAC have a bi-directional regulation of angiogenesis. A Representative images of the Oris™ Cell Migration Assay in EA.hy 926 cells. The EC-covered area was analyzed immediately following stopper removal (pre-migration, 0 h) or after drug treatment for 12 h. A detection area (Green Square) was defined in the same location for all wells, and the area covered with cells was determined under the indicated conditions. VEGF, PAl, or Sal C were promoted, while CA was inhibited during the cell migration after 12 h. B Quantitative PCR showing the expression of KDR, TIMP3, TSP1, and PECAM-1 in cells treated with PAl, Sal C, CA, or RA in ECs. C Representative Western blot analysis of CXCR4 expression (top) and densitometric quantitation (bottom). Compared with control, CXCR4 expression was significantly increased by PAl treatment but significantly decreased by CA and RA treatment. GAPDH was used as a loading control. ^*P < 0.05 vs. control group. n = 3. Data are presented as mean ± SEM