Fig. 4

Effects of CRT on the expression levels of CRT candidate targets involved in PI3K/AKT/NF-кB signaling in kidney tissues of DKD rats. A Representative western blots of various target proteins. B Relative protein expression levels of PI3K, p-PI3K, the ratio of p-PI3K to PI3K, AKT, p-AKT, the ratio of p-AKT to AKT, NF-кB (p65), p-NF-кB (p65) and the ratio of p-NF-кB (p65) to NF-кB (p65), respectively. C Expression and distribution of NF-кB (p65) protein in renal tissues of DKD rats. Original magnification, × 400. D Ratio of the number of cells with NF-кB (p65) nuclei translocation to the total number of cells, overlap coefficient and relative IOD value of NF-кB (p65) in nuclear in renal tissues cells of DKD rats. Data are shown as the mean ± SD. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, comparison with the CON group; ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, comparison with the DKD group