Fig. 5

Effects of CRT on the expression levels of CRT candidate targets involved in PI3K/AKT/NF-кB signaling, and the distribution of NF-кB (p65) protein in HK-2 cells treated with High Glucose. A, B Expression levels of IL-1β and TNF-α in serum and HK-2 cells treated with high glucose detected by ELISA. C Representative western blots. D Relative protein expression levels of PI3K, p-PI3K, the ratio of p-PI3K to PI3K, AKT, p-AKT, the ratio of p-AKT to AKT, NF-кB (p65), p-NF-кB (p65), the ratio of p-NF-кB (p65) to NF-кB (p65) in HK-2 cells treated with High Glucose. Data are shown as the mean ± SD. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, comparison with the CON group; *p < 0.05, **p < 0.01, ***p < 0.001, comparison with the HG group