Fig. 2

AR-CS inhibits the proliferation and migration of SW620 cells. Different concentrations of AR decoction (0.1, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 g/ml) treated SW620 cells for 12 h, CCK-8 assay was used to detect cell viability (A). Different concentrations of FBS (1%, 10%) or rat serum (RS) (5%, 10%, 20%) treated SW620 cells for 12 h, CCK-8 assay was used to detect cell viability (B). Different concentrations of AR-CS (0%, 20%, 40%, 80%, 100%) treated SW620 cells for 12 h, CCK-8 assay was used to detect cell viability (C). 20% AR-CS treated SW620 cells for 24 h, CCK-8 assay was used to detect cell viability in different administration time (0 h, 3 h, 6 h, 9 h, 12 h, 18 h, 24 h) (D). Different concentrations of AR-CS (0%, 20%, 10%, 5%) treated SW620 cells for 24 h, CCK-8 assay was used to detect cell viability (E), transwell assay (F) was used to detect cell migration rate (G), wound scratch assay (H) was used to detect cell wound healing rate (I). Scale bar = 100 µm in F, Scale bar = 400 µm in H. N = 10 in a–c, compared with 0% AR-CS treated group (control), *p < 0.05, **p < 0.01. N = 6 in d–g, compared with control group, **p < 0.01