Skip to main content
Fig. 3 | Chinese Medicine

Fig. 3

From: Astragalus membranaceus (Huangqi) and Rhizoma curcumae (Ezhu) decoction suppresses colorectal cancer via downregulation of Wnt5/β-Catenin signal

Fig. 3

AR-CS inhibits Wnt/β-Catenin signaling and EMT of SW620 cells. Different concentrations of AR-CS (0%, 20%, 10%, 5%) treated SW620 cells for 24 h, real-time qPCR was used to detect Wnt5 (A), β-Catenin (B), ARF6 (C), and N-Cadherin (D) mRNA expression. Western blot was used to detect Wnt5 and β-Catenin protein expression (E), Wnt5 (F) and β-Catenin (G) protein expression level was normalized to control group. Immunofluorescent staining was used to detect β-Catenin expression (H) and the fluorescence density was calculated (I). Scale bar = 20 µm. Western blot was used to detect ARF6 and N-Cadherin protein expression (J), ARF6 (K) and N-Cadherin (L) protein expression level was normalized to control group. N = 6, compared with the control group, **p < 0.01

Back to article page