Fig. 7

AR decoction inhibits tumor growth in nude mouse xenograft model. Intragastric administration of AR decoction (6.5 g/kg) for a nude mouse xenograft model was performed for 21 days. Oxaliplatin was used as a positive drug. The body weight of mice was weighed every two days (A). The tumor volume of mice was measured and calculated every three days (B). The tumors of mice (C) were taken and weighed (D) after administration of AR decoction. Western blot was used to detect Wnt5, β-Catenin, ARF6, N-Cadherin (E), LRP5, LRP6, TCF-4, and LEF1 (F) protein expression in tumors, the protein expression level was normalized to model group. HE staining was used to observe changes of organizational morphology in tumor (G), liver and kidney (H). Scale bar = 200 µm in G and H. Blood biochemical test was used to dectet Cr (I), BUN (J), AST (K), and ALT (L) levels in serum in mice. N = 5, compared with the model group, *p < 0.05