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Fig. 1 | Chinese Medicine

Fig. 1

From: Toddalolactone protects against osteoarthritis by ameliorating chondrocyte inflammation and suppressing osteoclastogenesis

Fig. 1

TOD inhibited the expression of inflammatory and catabolic mediators in LPS-induced chondrocytes. A Chemical structure of TOD. B The effect of TOD on the cell viability of SW1353 was evaluated by CCK-8. C Gradient concentrations (0, 0.5, 1, 5, 10, 20, 40 µM/ml) of TOD on the apoptosis level of SW1353 was measured by flow cytometry. D Western blotting performed after SW1353 cells were treated with gradient concentrations (0, 5, 10, and 20 µM/ml) of TOD; β-Tubulin was used as the loading control. E Pro-inflammatory cytokines, including IL-6, IL-8, TNF-α, MMP2, MMP9, and MMP13 were measured by RT-PCR. F ELISA was used to measure the expression level of MMP2, MMP9, MMP13, IL-6, and TNF-α. Data represent means ± SD of triplicate independent experiments. *, # indicates p < 0.05, **, ## indicates p < 0.01, ns indicates not significant

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