Fig. 3

TOD inhibits the formation of F-actin ring. A BMMs were cultured with M-CSF (25 ng/ml), RANKL (50 ng/ml), and the indicated concentrations (0, 5, 10, and 20 µM) of TOD for 5 days. After differentiation, BMMs were fixed and stained for F-actin ring. Scale bar: 100 μm. B The size per F-actin ring and area of osteoclasts were quantified. C BMMs were cultured with M-CSF (25 ng/ml) and RANKL (50 ng/ml) for 5 days, and TOD (20 µM) was added at different stages during osteoclast differentiation. After differentiation, BMMs were fixed and stained for F-actin ring. Scale bar: 100 μm. D The size per F-actin ring and area of osteoclasts were quantified. Data represent means ± SD of triplicate independent experiments. ^{*, #} indicates p < 0.05, ^{**, ##} indicates p < 0.01, ^ns indicates not significant