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Fig. 5 | Chinese Medicine

Fig. 5

From: Toddalolactone protects against osteoarthritis by ameliorating chondrocyte inflammation and suppressing osteoclastogenesis

Fig. 5

TOD inhibits the activation of the NF-κB and MAPK signalling pathways in SW1353 cells. A Western blotting performed the activity of NF-κB and MAPK signalling pathways in SW1353 cells; GAPDH was used as the loading control. B ImageJ software was used to quantify the density of the western blot bands (P-P65, IκBα, P-P38, and P-JNK) shown in A. C Western blots performed the protein expression of P65 in the nucleus and cytoplasm in SW1353 cells. D ImageJ software was used to quantify the density of the western blot bands in C. E Immunofluorescence was used to determine nuclear translocation of p65 in SW1353 cells. Scale bar: 100 μm. Data represent means ± SD of triplicate independent experiments. *, # indicates p < 0.05, **, ## indicates p < 0.01, ns indicates not significant

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