Fig. 7

TOD protects against ACLT-induced cartilage destruction and inhibits osteoclastogenesis in vivo. A H&E staining, Safranin O/Fast Green staining, Toluidine Blue, and TUNEL DAPI/FITC staining were performed respectively. Scale bar: 100 μm. B Immunohistochemical analysis of Collagen II. Scale bar: 100 μm (upper); 10 μm (lower). C Immunohistochemical analysis of P65. Scale bar: 100 μm (upper); 10 μm (lower). D TRAP staining of the osteoclasts in the subchondral plate. Scale bar: 200 μm. E The OARSI scores of different groups were assessed. F The percentage of apoptotic chondrocytes in cartilage was calculated. G Quantitative analysis of the immunohistochemical staining of Collagen II. H Quantitative analysis of the immunohistochemical staining of P65. Data represent means ± SD of triplicate independent experiments. ^{*, #} indicates p < 0.05, ^{**, ##} indicates p < 0.01, ^ns indicates not significant