Fig. 5

TFA increased the expression of conjunct proteins in brain hippocampus tissue. A Western Blot of ZO-1 and claudin 5. B Immunofluorescence of ZO-1 protein in Bend.3 cell induced by AGEs (AGEs-L: 100 μg/ml, AGEs-H: 200 μg/ml). C Cell viability tested by MTT. D ZO-1, E Claudin 5, F PGC-1α, G p-AMPK and AMPK proteins expression tested by immunofluorescence in Bend.3 cells; H and I relative protein fluorescence density analysis by Image J. AGEs: Advanced Glycation End products. *p < 0.05, **p < 0.01, vs. NC group or vehicle group; ^#p < 0.05, ^##p < 0.01, vs. T2DM group or AGEs group