Fig. 3From: Cucurbitacin B inhibits TGF-β1-induced epithelial–mesenchymal transition (EMT) in NSCLC through regulating ROS and PI3K/Akt/mTOR pathwaysCuB reversed TGF-β1-induced EMT in A549 cells through PI3K/Akt/mTOR pathway. a Western blotting assay detect the proteins expression of E-cadherin, N-cadherin, Vimentin and ZEB1 in A549 cells after co-treated with CuB and TGF-β1 for 48 h. b–e The statistic results of protein expression of a, ###P < 0.001 vs control group, ***P < 0.001 vs TGF-β1 group. f, g Immunofluorescence of E-cadherin and N-cadherin in A549 cells after co-treated with CuB (15 nM) and TGF-β1 (4 ng/mL) for 48 h (Scale bar = 7.5 μm)Back to article page