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Fig. 6 | Chinese Medicine

Fig. 6

From: Cucurbitacin B inhibits TGF-β1-induced epithelial–mesenchymal transition (EMT) in NSCLC through regulating ROS and PI3K/Akt/mTOR pathways

Fig. 6

CuB reversed EMT in Gefitinib resistant A549 cells via ROS and PI3K/Akt/mTOR pathway. a The morphology of A549 cells and A549 Gefitinib resistant (A549-GR) cells. b The resistant index of A549-GR cells compared to A549 cells. c The immunofluorescence of EGFR in A549-GR cells after treatment with CuB (15 nM) or Gefitinib (10 μM) for 48 h (Scale bar = 7.5 μm). d The inhibition effect of CuB (15 nM) or Gefitinib (10 μM) in A549-GR cells was detected by wound healing assay at 0 h and 48 h. e, f Transwell assay was used to detect the inhibition effect of CuB (15 nM) or Gefitinib (10 μM) on migration and invasion ability in A549-GR cells after treatment for 48 h (Scale bar = 25 μm). gi The statistic results of Fig. 7d-f. ###P < 0.001 vs A549 cells, *P < 0.05, ***P < 0.001 vs A549-GR cells

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