Fig. 3

HSDL2 promotes the growth of melanoma in vitro and in vivo. HSDL2 was overexpressed or silenced in A375 cells, and A CCK-8 assays were performed. B Colony forming assays were performed. Imaging and quantification of colony formation assays were performed between HSDL2-silenced A375 cells or HSDL2-overexpressing A375 cells and control cells. C Apoptotic cells were tested by FACS analysis using an Annexin V-FITC Apoptosis Detection Kit. D Apoptosis markers (BaX, Bcl-2 and cleaved-Caspase3) were detected by western blotting. E–G A375 cells with silenced HSDL2 were subcutaneously injected into nude mice. After 4 weeks, the mice were sacrificed and dissected at the endpoint. Tumour growth and weight were examined (n = 6/group). The experiments were repeated three times. All data represent the mean ± SEM, *P < 0.05, **P < 0.01 and ***P < 0.001, compared with the control group