Fig. 4

HSDL2 promotes melanoma through the ERK and AKT signalling pathways. A375 cells were infected with shCtrl or shHSDL2 lentivirus. A Phosphorylation of 17 signalling proteins was analysed with the PathScan Stress and Apoptosis Signaling Antibody Array, and the image pixel intensity ratio of signalling molecules is shown. B HSDL2 was overexpressed in A375 cells, and the ERK and AKT signalling pathways were determined by western blotting. C HSDL2 was silenced in A375 cells, and the ERK and AKT signalling pathways were examined by western blotting. Data represent one of three independent experiments. **P < 0.01, compared with the control group