Fig. 5

CuE inhibits HSDL2 expression. A375 cells were treated with different concentrations of CuE, and the mRNA and protein levels were examined by RT-qPCR (A) and western blotting (B). The experiments were repeated three times, and the results of other two repeated experiments on western blotting about HSDL2 expression were showed in Additional file 1: Fig. S1. All data represent the mean ± SEM, *P < 0.05, **P < 0.01 and ***P < 0.001, compared with the 0 μM CuE treatment group