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Fig. 7 | Chinese Medicine

Fig. 7

From: Cucurbitacin E inhibits cellular proliferation and induces apoptosis in melanoma by suppressing HSDL2 expression

Fig. 7

CuE slows melanoma cell growth by mediating HSDL2. A375 cells with or without HSDL2 overexpression were treated with CuE, and the mRNA and protein levels of HSDL2 were tested by RT–qPCR and western blotting (A). The proliferation-inhibiting ability of CuE in HSDL2-overexpressing A375 cells was analysed by CCK-8 assay (B) and colony forming assay (C). The apoptosis of A375 cells with or without HSDL2 overexpression treated with CuE was analysed by flow cytometry (D). The levels of Bcl2, BaX and cleaved caspase-3 in A375 cells with or without HSDL2 overexpression treated with CuE were analysed by western blotting (E). A375 cells with or without HSDL2 overexpression were subcutaneously injected into nude mice, and then the tumour-bearing mice were intraperitoneally injected with CuE. After 4 weeks, the mice were sacrificed and dissected at the endpoint. Tumour growth and weight were examined (n = 6/group, FH). Experiments were repeated three times. All data represent the mean ± SEM, *P < 0.05, **P < 0.01 and ***P < 0.001, compared with the control group

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