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Table 1 Properties of CRRISPR-Cas9 orthologs

From: CRISPR-Cas gene editing technology and its application prospect in medicinal plants

Cas9 orthologs Native bacteria PAM (5′ to 3′) Size (amino acids) Refs.
SpCas9 Streptococcus pyogenes Cas9 NGG 1368 [44, 45]
SaCas9 Staphylococcus aureus Cas9 NNGRRT 1053 [45, 46]
ScCas9 Streptococcus canis Cas9 NNG 1375 [47]
NmCas9 Neisseria meningitidis Cas9 NNNNGATT 1082 [43, 48]
CjCas9 Campylobacter jejuni Cas9 NNNNRYAC; NNNNACAC 984 [49, 50]
St1Cas9 Streptococcus thermophilus CRISPR1 Cas9 NNAGAAW 1121 [51]
St3Cas9 Streptococcus thermophilus CRISPR3 Cas9 NGGNG 1388 [51]
FnCas9 Francisella novicida Cas9 NGG 1629 [52, 53]
TdCas9 Treponema denticola Cas9 NAAAAN 1423 [54]
SmacCas9 Streptococcus macacae Cas9 NAA 1338 [55]
BlatCas9 Brevibacillus laterosporus SSP360D4 Cas9 NNNNCND 1092 [56]
CasX Deltaproteobacteria and Planctomycetes phyla TTCN 980 [45, 57, 58]
CasY Katanobacteria, Vogelbacteria, Parcubacteria, Komeilibacteria and Kerfeldbacteria TA 1200 [47, 59]