CRISPR-Cas gene editing technology and its application prospect in medicinal plants

Chinese Medicine

Table 1 Properties of CRRISPR-Cas9 orthologs

Cas9 orthologs	Native bacteria	PAM (5′ to 3′)	Size (amino acids)	Refs.
SpCas9	Streptococcus pyogenes Cas9	NGG	1368	[44, 45]
SaCas9	Staphylococcus aureus Cas9	NNGRRT	1053	[45, 46]
ScCas9	Streptococcus canis Cas9	NNG	1375	[47]
NmCas9	Neisseria meningitidis Cas9	NNNNGATT	1082	[43, 48]
CjCas9	Campylobacter jejuni Cas9	NNNNRYAC; NNNNACAC	984	[49, 50]
St1Cas9	Streptococcus thermophilus CRISPR1 Cas9	NNAGAAW	1121	[51]
St3Cas9	Streptococcus thermophilus CRISPR3 Cas9	NGGNG	1388	[51]
FnCas9	Francisella novicida Cas9	NGG	1629	[52, 53]
TdCas9	Treponema denticola Cas9	NAAAAN	1423	[54]
SmacCas9	Streptococcus macacae Cas9	NAA	1338	[55]
BlatCas9	Brevibacillus laterosporus SSP360D4 Cas9	NNNNCND	1092	[56]
CasX	Deltaproteobacteria and Planctomycetes phyla	TTCN	980	[45, 57, 58]
CasY	Katanobacteria, Vogelbacteria, Parcubacteria, Komeilibacteria and Kerfeldbacteria	TA	1200	[47, 59]

ISSN: 1749-8546