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Fig. 6 | Chinese Medicine

Fig. 6

From: Cinnamomi Ramulus inhibits the growth of colon cancer cells via Akt/ERK signaling pathways

Fig. 6

CR treatment resulted in apoptosis and disturbance of cell cycle progression in CC cells. A The cell morphology was observed in white light and fluorescence field using an inverted microscope. B Induction of apoptosis of HT-29 and HCT-15 cells after CR treatment for 24 h. The apoptotic events was assessed by flow cytometry. C and D Statistical analysis of the percentages of the apoptotic cells in HT-29 and HCT-15 cells. *p < 0.05, **p < 0.01 and ***p < 0.001 based on the Student's t-test. E Cell cycle analysis of HT-29 and HCT-15 cells following CR treatment for 24 h by flow cytometry. F and G Statistical analysis of the proportions of the cells at different phases in HT-29 and HCT-15 cells. *p < 0.05 and ***p < 0.001 based on the Student's t-test. H HT-29 and HCT-15 cells were treated with CR at different concentrations for 24 h. After proteins were extracted, the expression levels of Bcl-2, Bax, pro-caspase-9, cleaved-caspase-9, pro-PARP, cleaved-PARP, Cyclin D1, CDK2, CyclinA2 and CyclinB1 were analyzed by WB assay, respectively

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