Fig. 8

Five main ingredients obtained from CR by using UPLC-MS/MS and relevant analyses. A (i) The ultraviolet absorption wavelength map. (ii) The total ion chromatography (TICs) in positive ion modes. (iii) The total ion chromatography (TICs) in negative ion modes. B The five main ingredients were identified by secondary mass spectrometry. C The Venn diagram of 9 common key core targets from CR-related targets and candidate core targets. D Binding modes from AutoDock 4.2. Taxifolin with HSP1A1, HSP90AB1 and PARP1, respectively. E CCK-8 assay was performed to measure cell viability at 24 h and 48 h after taxifolin treatment at different dosages (0, 0.05, 0.1, 0.15, 0.2, 0.3 mg/mL). F HT-29 and HCT-15 cells were treated with taxifolin at different concentrations for 24 h. After proteins were extracted, the expression levels of pro-PARP, cleaved-PARP, p-Akt (T308), p-Akt (S473), pan-Akt, p-ERK (T202/Y204) and pan-ERK were analyzed by WB assay, respectively