Fig. 4

α-mangostin attenuates bone resorptive activity of mature osteoclasts and inhibits F‐actin ring formation in vitro. Same number of BMM‐derived mature osteoclasts were seeded onto bovine bone slices and were treated with the indicated α-mangostin concentrations for 2 days. A The cells were stained with rhodamine‐conjugated phalloidin and representative fluorescence microscope images of F‐actin rings.are shown. scale bar = 20 μm. B Representative images of bone resorption pits were acquired by scanning electron microscopy (SEM); scale bar = 100 μm. C The resorption area of bone discs and the size of F-actin rings were quantified using the ImageJ software, N = 3. Error bar = mean ± SD. **P < 0.01, ***P < 0.001, compared with the controls