Fig. 3

AGNHW treatment preserved the BBB integrity, attenuated brain edema and decreased hemorrhagic transformation (HT) in transient ischemic stroke with delayed t-PA treatment. A Evans blue leakage assay was used to assess the severity of BBB injury in the ischemic hemispheres at 24 h after MCAO. n = 5. B Quantitative analysis of brain edema: The brain edema was quantified by dividing the total area of ischemic side by the total area of non-ischemic side at 24 h after MCAO. n = 10. C Representative HT slices from ischemic brains. D Quantitative assessment of hemoglobin levels in the ischemic brains to determine HT severity, n = 6. E hemorrhagic scores HT scores as another measure for HT, *P < 0.05, *** P < 0.001, n = 10 per group