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Fig. 5 | Chinese Medicine

Fig. 5

From: Angong Niuhuang Wan reduces hemorrhagic transformation and mortality in ischemic stroke rats with delayed thrombolysis: involvement of peroxynitrite-mediated MMP-9 activation

Fig. 5

AGNHW treatment inhibited peroxynitrite and superoxide fluorescence in cultured brain microvascular endothelial b.End3 cells in vitro. Ctr, cells were incubated under normoxic condition; OGD, cells were exposed to 5 h of oxygen and glucose deprivation followed by 5 h of reoxygenation; Ctr + t-PA, cells were treated with t-PA (20 µg/ml) under normoxic condition; OGD + t-PA, cells were exposed to 5 h of oxygen and glucose deprivation followed by 5 h of reoxygenation, and the cells were treated with t-PA (20 µg/ml) upon reoxygenation; OGD + t-PA + AGNHW: Cells were subjected to 5 h of oxygen and glucose deprivation 5 h of reoxygenation before being co-treated with t-PA (20 µg/ml) and AGNHW (50, 100 µg/ml) upon reoxygenation. HKYellow AM and HEt staining were used to detect ONOO and O2.−, respectively. A Representative fluorescent image of HEt staining for O2.− in b.End3 cells. B Representative fluorescent image of HKYellow AM staining for ONOO in b.End3 cells in vitro

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