Table 1 Primer sequence for RT-qPCR
Target gene | Direction | Sequence (5′-3′) |
|---|---|---|
IFN-α (mouse) | Fwd | ACTCATTCTGCACTGGCCTCCA |
| Â | Rev | ACTTCTGCTCTGACCACCTCCC |
IFN-γ (mouse) | Fwd | TGACATGAAAATCCTGCAGAGCC |
| Â | Rev | GCTGGACCTGTGGGTTGTTGAC |
IP-10 (mouse) | Fwd | TGAGGGCCATAGGGAAGCTTGAAAT |
| Â | Rev | TCCGGATTCAGACATCTCTGCTCAT |
MCP-1 (mouse) | Fwd | AAGATCTCAGTGCAGAGGCTCG |
| Â | Rev | CCA GGGGTAGAACTGTGGTTCAA |
GAPDH(mouse) | Fwd | CAAAATGGTGAAGGTCGGTGTG |
| Â | Rev | GTTGAGGTCAATGAAGGGGTCG |
IL-6 (human) | Forward | CGGGAACGAAAGAGAAGCTCTA |
| Â | Reverse | CGCTTGTGGAGAAGGAGTTCA |
CCL-5 (human) | Forward | CAGCAGTCGTCTTTGTCACC |
| Â | Reverse | GTTGATGTACTCCCGAACCC |
IL-8 (human) | Forward | CTTGGTTTCTCCTTTATTTCTA |
| Â | Reverse | GCACAAATATTTGATGCTTAA |
IP-10 (human) | Forward | GAAATTATTCCTGCAAGCCAATTT |
| Â | Reverse | TCACCCTTCTTTTTCATTGTAGCA |
IL-1α (human) | Forward | GAAGATGTGCCTGTCCTGTGT |
| Â | Reverse | CGCTCAGGTCAGTGATGTTAA |
MIP-1α (human) | Forward | CTGCATCACTTGCTGCTGACA |
| Â | Reverse | CACTGGCTGCTCGTCTCAAAG |
GAPDH(human) | Forward | GAAGGTGAAGGTCGGAGTC |
| Â | Reverse | GAAGATGGTGATGGGATTTC |