Fig. 6

TSN inhibited autophagic flux and repressed TNBC xenograft growth in vivo. A Images showed the tumors tissues isolated from mice receiving vehicle, irinotecan (10 mg/kg), TSN (0.5 mg/kg) or SN-38 a combination of irinotecan and TSN treatment. B Body weight changes of mice during 28 days of exposure. C Average tumor volume calculation. Error bars represent means ± SD. D Tumor weight measurement at day 28. E Tumor tissues were sectioned and stained with hoechst. The fluorescent images were taken under a confocal microscope. F Western blot analysis of LC3-II and SQSTM1 in tumor tissues (n = 3) and the densitometric analysis of LC3-II and SQSTM1 in tumor tissues. G Tumor tissues were sectioned and subjected to immunostaining of cleaved caspase 3 for evaluating apoptosis. Scale bars: 100 μm. The results are expressed as the mean ± SD values obtained from three independent experiments (*p < 0.05, **p < 0.01, ***p < 0.001 vs. irinotecan alone; ^##p < 0.01, ^###p < 0.001 vs. CTL)