Table 3 Global semi-quantitative analysis of the 47 common components identified in three SHL preparation forms
From: Metabolomics analyses of traditional Chinese medicine formula Shuang Huang Lian by UHPLC-QTOF-MS/MS
No | Formula | Name | Intra-day (n = 3) | Inter-day (n = 3) | ||||
|---|---|---|---|---|---|---|---|---|
G ± SD (CV%) (µgb) | O ± SD (CV%) (µgb) | T ± SD (CV%) (µgb) | G ± SD (CV%) (µgb) | O ± SD (CV%) (µgb) | T ± SD (CV%) (µgb) | |||
1 | C21H20O13 | Tagetiin | 44 ± 8 (18) | 61 ± 3 (5) | 101 ± 5 (5) | 49 ± 10 (20) | 60 ± 3 (5) | 103 ± 4 (4) |
2 | C28H16O5 | Naphthofluorescein | 27 ± 7 (26) | 39 ± 14 (37) | 76 ± 4 (6) | 41 ± 13 (31) | 43 ± 12 (28) | 75 ± 3 (5) |
3 | C21H26N4O8 | Trp-Glu-Glu | 52 ± 4 (8) | 53 ± 3 (5) | 28 ± 3 (11) | 55 ± 7 (12) | 57 ± 6 (11) | 30 ± 3 (11) |
4 | C9H6O3 | Umbelliferone | 31 ± 1 (3) | 26 ± 2 (7) | 54 ± 3 (6) | 31 ± 2 (5) | 25.3 ± 0.6 (2) | 61 ± 7 (11) |
5 | C16H18O9 | Chlorogenic Acida | 207 ± 19 (9) | 143 ± 15 (11) | 305 ± 31 (10) | 200 ± 19 (9) | 142 ± 10 (7) | 299 ± 43 (15) |
6 | C10H10O4 | Methyl caffeate | 2.52 ± 0.09 (3) | 42 ± 3 (7) | 33 ± 3 (10) | 2.55 ± 0.03 (1) | 43 ± 3 (7) | 32.7 ± 0.6 (2) |
7 | C10H12O5 | Danielone | 5.3 ± 0.1 (2) | 79 ± 10 (13) | 71 ± 3 (4) | 5.42 ± 0.08 (1) | 79 ± 5 (6) | 70 ± 5 (6) |
8 | C8H6O3 | Piperonal | 2.05 ± 0.05 (3) | 37 ± 4 (12) | 32 ± 4 (11) | 1.8 ± 0.3 (15) | 39 ± 6 (14) | 32 ± 3 (9) |
9 | C16H22O10 | Geniposidic acid | 8.6 ± 0.2 (2) | 127 ± 3 (3) | 72 ± 7 (10) | 8.3 ± 0.7 (8) | 130 ± 5 (4) | 70 ± 6 (9) |
10 | C16H18O8 | p-Coumaroyl quinic acid | 51 ± 7 (14) | 4 ± 1 (32) | 11 ± 1 (9) | 49 ± 6 (12) | 4 ± 1 (38) | 11 ± 1 (9) |
11 | C16H22O9 | Tarennoside | 3.9 ± 0.2 (4) | 82.0 ± 0.8 (1) | 241.00 ± 0.07 (0.03) | 4.0 ± 0.1 (3) | 82 ± 1 (1) | 239 ± 3 (1) |
12 | C15H26N6O6 | Asp-Arg-Pro | 40 ± 4 (11) | 63 ± 12 (19) | 91 ± 7 (8) | 51 ± 18 (36) | 66 ± 10 (15) | 89 ± 6 (7) |
13 | C16H28N6O8 | Arg-Glu-Glu | 8 ± 1 (14) | 11.8 ± 0.7 (6) | 1.3 ± 0.1 (9) | 7 ± 2 (29) | 11.8 ± 0.7 (6) | 1.3 ± 0.1 (9) |
14 | C10H12O4 | Paeonilactone B | 2.79 ± 0.03 (1) | 51.25 ± 0.09 (0.2) | 136 ± 6 (4) | 2.78 ± 0.03 (1) | 51.0 ± 0.4 (1) | 137 ± 5 (3) |
15 | C20H27N5O6 | Thr-Gln-Trp | 43 ± 4 (10) | 42 ± 7 (16) | 64 ± 2 (3) | 46 ± 6 (13) | 45 ± 8 (17) | 63 ± 3 (5) |
16 | C20H24N4O6 | Pro-Trp-Asp | 34 ± 4 (12) | 33 ± 6 (19) | 55 ± 2 (3) | 35 ± 3 (8) | 32 ± 5 (15) | 54 ± 2 (3) |
17 | C15H21N5O8 | Asp-Glu-His | 25 ± 1 (4) | 35 ± 1 (4) | 36 ± 5 (15) | 27 ± 4 (13) | 35 ± 1 (4) | 34 ± 5 (16) |
18 | C16H18N6O4 | 2-Phenylaminoadenosine | 110 ± 11 (10) | 36 ± 6 (17) | 108 ± 2 (2) | 110 ± 11 (10) | 37 ± 5 (14) | 107 ± 2 (1) |
19 | C27H30O16 | Rutin | 316.4 ± 0.6 (0.2) | 39 ± 4 (9) | 55 ± 1 (2) | 321 ± 8 (2) | 39 ± 2 (6) | 55 ± 1 (2) |
20 | C21H18O12 | Luteolin 3′-glucuronide | 107 ± 10 (9) | 122 ± 6 (5) | 111 ± 11 (10) | 128 ± 18 (14) | 109 ± 15 (14) | 131 ± 20 (15) |
21 | C21H20O11 | Luteolin-7-O-glucosidea | 64 ± 4 (6) | 178 ± 3 (2) | 294 ± 6 (2) | 62 ± 2 (3) | 178.4 ± 0.8 (0.5) | 285 ± 9 (3) |
22 | C21H26O12 | Plumieride | 324 ± 17 (5) | 462 ± 8 (2) | 200 ± 11 (6) | 327 ± 7 (2) | 471 ± 11 (2) | 199 ± 11 (6) |
23 | C29H36O15 | Forsythoside Aa | 290 ± 24 (8) | 398 ± 12 (3) | 172 ± 2 (1) | 285 ± 9 (3) | 414 ± 17 (4) | 171 ± 1 (0.4) |
24 | C13H28N6O8 | Zwittermicin A | 5.8 ± 0.3 (5) | 28 ± 2 (6) | 1.3 ± 0.1 (11) | 6 ± 1 (16) | 22 ± 5 (20) | 1.5 ± 0.2 (16) |
25 | C20H20O5 | Morachalcone A | 98.8 ± 0.3 (0.3) | 97 ± 9 (10) | 66 ± 2 (3) | 98.7 ± 0.3 (0.3) | 99 ± 7 (8) | 64 ± 4 (6) |
26 | C26H32O11 | Brusatol | 258 ± 14 (5) | 227 ± 10 (4) | 164 ± 2 (1) | 261 ± 2 (1) | 226 ± 5 (2) | 164 ± 2 (1) |
27 | C27H30O14 | Isofurcatain 7-O-glucoside | 2.7 ± 0.3 (10) | 85.7 ± 0.4 (0.5) | 178 ± 2 (1) | 2.7 ± 0.2 (8) | 85.8 ± 0.3 (0.4) | 175 ± 4 (2) |
28 | C25H24O12 | Apigenin 7-(3″,4″-diacetylglucoside) | 14.3 ± 0.1 (1) | 92 ± 1 (1) | 98 ± 2 (2) | 14.3 ± 0.1 (1) | 91.4 ± 0.9 (1) | 98 ± 1 (1) |
29 | C21H20O10 | Isovitexin | 256 ± 17 (6) | 162 ± 1 (0.6) | 138 ± 7 (5) | 257 ± 11 (4) | 161 ± 2 (2) | 138 ± 5 (3) |
30 | C27H34O11 | Undulatone | 23 ± 2 (7) | 82 ± 6 (8) | 91 ± 25 (28) | 23 ± 2 (7) | 84 ± 6 (7) | 91 ± 25 (27) |
31 | C21H18O11 | Baicalina | 62 ± 2 (4) | 30 ± 3 (9) | 229 ± 17 (8) | 56 ± 5 (8) | 32 ± 2 (5) | 221 ± 9 (4) |
32 | C27H34O11 | Forsythina | 1051 ± 90 (9) | 463 ± 17 (4) | 898 ± 31 (3) | 1056 ± 25 (2) | 463 ± 11 (2) | 895 ± 26 (3) |
33 | C22H20O12 | Hispidulin 7-glucuronide | 20 ± 2 (12) | 12.17 ± 0.05 (0.4) | 59 ± 3 (5) | 21 ± 2 (10) | 12.16 ± 0.04 (0.4) | 58 ± 3 (4) |
34 | C21H18O10 | Chrysin 7-glucuronide | 376 ± 25 (7) | 306 ± 12 (4) | 461 ± 22 (5) | 382 ± 20 (5) | 308 ± 10 (3) | 456 ± 18 (4) |
35 | C22H20O11 | Wogonin 7-glucuronide | 888 ± 75 (8) | 840 ± 52 (6) | 1278 ± 37 (3) | 905 ± 61 (7) | 852 ± 42 (5) | 1268 ± 31 (2) |
36 | C21H18O11 | Apigenin 7-glucuronide | 47 ± 2 (4) | 21.9 ± 0.8 (3) | 86 ± 1 (1) | 48 ± 2 (3) | 21.9 ± 0.5 (2) | 85 ± 2 (2) |
37 | C16H12O6 | Kaempferide | 22 ± 1 (4) | 34.6 ± 0.1 (0.4) | 37.2 ± 0.3 (1) | 21.7 ± 0.7 (3) | 34.2 ± 0.4 (1) | 36 ± 2 (4) |
38 | C15H10O5 | Baicalein | 275 ± 14 (5) | 212 ± 4 (2) | 264 ± 26 (10) | 278 ± 6 (2) | 213 ± 5 (2) | 271 ± 10 (4) |
39 | C21H24O6 | Kadsurin A | 50.1 ± 0.7 (1) | 20 ± 1 (6) | 31 ± 1 (3) | 50.2 ± 0.5 (1) | 19 ± 1 (5) | 31.6 ± 0.8 (2) |
40 | C16H12O5 | Wogonin | 119 ± 6 (5) | 161 ± 4 (3) | 169 ± 1 (1) | 118 ± 3 (2) | 161 ± 3 (2) | 166 ± 3 (2) |
41 | C17H14O6 | 5,3′-Dihydroxy-7,4′-dimethoxy-4-phenylcoumarin | 12.8 ± 0.7 (5) | 22.3 ± 0.8 (4) | 28 ± 1 (5) | 12 ± 1 (8) | 21 ± 2 (7) | 27 ± 3 (10) |
42 | C19H18O8 | Skullcapflavone II | 27 ± 2 (9) | 13 ± 1 (8) | 73 ± 6 (8) | 28 ± 1 (4) | 13.0 ± 0.7 (5) | 72 ± 4 (6) |
43 | C15H22O2 | Eremophilenolide | 4.4 ± 0.3 (7) | 4.6 ± 0.5 (10) | 6.5 ± 0.6 (9) | 4.4 ± 0.2 (5) | 4.7 ± 0.4 (8) | 6.3 ± 0.5 (7) |
44 | C24H50NO7P | PE (19:0/0:0) | 147 ± 16 (11) | 13.9 ± 0.4 (3) | 99 ± 5 (5) | 150 ± 13 (9) | 14.0 ± 0.3 (2) | 98 ± 4 (4) |
45 | C19H38O4 | 1-Monopalmitin | 13 ± 2 (12) | 11 ± 1 (10) | 17.4 ± 0.9 (5) | 13 ± 1 (9) | 11.1 ± 0.9 (8) | 17.2 ± 0.7 (4) |
46 | C51H84O15 | 1,2-Di-(9Z,12Z,15Z-octadecatrienoyl)-3-(galactosyl-alpha-1-6-galactosyl-beta-1)-glycerol | 88 ± 3 (3) | 16.8 ± 0.9 (5) | 75 ± 1 (2) | 89 ± 2 (2) | 16.6 ± 0.7 (4) | 74 ± 1 (1) |
47 | C45H74O10 | 1,2-Di-(9Z,12Z,15Z-octadecatrienoyl)-3-O-Beta-d-galactosyl-sn-glycerol | 68 ± 6 (9) | 54.8 ± 0.4 (1) | 62 ± 4 (6) | 69 ± 5 (8) | 58 ± 6 (11) | 58 ± 7 (12) |