Fig. 3

Electroacupuncture reduced the expression of lncRNA Miat. A Schematic illustration of the genomic locus of Miat and neighboring genes on rat chromosome 12q16. B Miat was predicted to be of a poor protein coding capacity. The RNA sequences of Miat, MALAT1, Actb and Gapdh were evaluated by the CNIT program. MALAT1 served as a control non-coding transcript, while Gapdh and Actb served as control protein coding transcripts. C Subcellular localization of Miat precited by the lncLocator program. D Nuclear separation experiments showed the subcellular localization of Miat. Actin mRNA was used as a control for the cytoplasmic fraction; U6 mRNA was used as a control for the nuclear fraction. E The relative expression levels of Miat were detected in different groups. Values are expressed as the means ± SD (n = 3), *P < 0.05, **P < 0.01