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Fig. 4 | Chinese Medicine

Fig. 4

From: Downregulation of lncRNA Miat contributes to the protective effect of electroacupuncture against myocardial fibrosis

Fig. 4

Miat sponges miR-133a in an Ago2-dependent manner. A, B Bioinformatics analysis of the possible binding sites between Miat and miR-133a. C The RIP assay and Western blot were performed to confirm whether Miat and miR-133a-3p could directly bind to Ago2 in H9c2 cells. D Anti-Ago2 RIP was performed in H9c2 cells transiently overexpressing miR-133a and followed by RT-qPCR and western blot to detect lncRNA-Miat associated with Ago2. Values are expressed as the means ± SD (n = 3), *P < 0.05, **P < 0.01

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