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Fig. 5 | Chinese Medicine

Fig. 5

From: Downregulation of lncRNA Miat contributes to the protective effect of electroacupuncture against myocardial fibrosis

Fig. 5

CTGF is a direct target of miR-133a-3p in H9c2 cells. A CTGF was predicted as a target gene of miR-133a-3p using Targetscan. B HEK293 cells were transfected with miR-133a-3p mimic and luciferase constructs of CTGF 3′-UTR (Wild Type, WT) or mutant CTGF 3′-UTR-MUT. Luciferase activity was detected 48 h after transfection. C, D H9c2 cells were transfected with miR-133a-3p mimic. The mRNA (C) and protein (D) expression levels of CTGF were determined by RT-qPCR and Western blot. E A schematic outline of how the lncRNA Miat may function as a ceRNA for miR-133a-3p-Ago2 and promote the expression of CTGF protein. Values are expressed as the means ± SD (n = 3), *P < 0.05, **P < 0.01

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