Fig. 7

Miat inhibits the formation of the heterodimer PPARG–RXRA complex and promotes the transcription of TGF-β1. A Relative expression of Miat determined by RT-qPCR in H9c2 cells transfected with pcDNA3.1 or Miat overexpression vector. B RT-qPCR confirmed the down-regulation of Miat using two different siRNAs compared with the negative control (NC). C The dual-luciferase reporter assay detected the transcriptional activity of TGF-β1 with Miat overexpressing and knockdown. The values were normalized to control expression levels (n = 3). D Co-IP experiment was used to detect the binding between PPARG and RXRA with or without Miat overexpression. E Schematic illustration of the mechanism by which lncRNA-Miat acts as a decoy to weaken PPARG/RXRA interactions, inhibit TF (for example Zf9) dephosphorylation and promote the transcriptions of TGF-β1. Values are expressed as the means ± SD (n = 3), *P < 0.05, **P < 0.01