Fig. 3

AP delays anagen-catagen transition. a Experimental scheme of topical drug treatment and sampling, n = 5 mice per group. b H&E staining of VC-, PC- and AP-treated skin at D18, D19, and D22 after depilation. n = 5 mice per group. Scale bars, 100 μm for D18, D19, 50 μm for D22. c Immunofluorescence images and quantification of EdU staining within hair follicles of VC-, PC-, and AP-treated mice at D19 after depilation. n = 50 hair follicles per group from 5 mice. Scale bars, 50 μm. d Immunofluorescence images and quantification of capspase3 staining within hair follicles of VC-, PC-, and AP-treated mice at D19 after depilation. n = 50 hair follicles per group from 5 mice. Scale bars, 50 μm. e Immunofluorescence images of K15 staining in hair follicles of VC-, PC-, and AP-treated mice at D19 after depilation. n = 5 mice per group. Scale bars, 100 μm. f Quantification of DP size of VC-, PC-, and AP-treated mice at D19 after depilation. g Comparison of the duration of catagen, anagen and telogen phase in VC-, PC- and AP-treated skin, n = 5 mice per group