Fig. 4

AP stimulates Lgr5+ HFSC at the onset of anagen. a Upper panel: experimental scheme of topical drug treatment and sampling. Lower panel: immunofluorescence images for dual-staining of EdU and Lef1, dual-staining of EdU and K15 in hair follicles of VC-, PC-, and AP-treated mice at D4 after depilation. Quantification of K15 staining in hair follicles (n = 50 hair follicles per group from 5 mice). b Upper panel: experimental scheme of topical drug treatment and sampling. Lower panel: lineage tracing of Lgr5+ HFSCs by using Lgr5^{EGFP−CreERT2}; R26R^tdTomato mice at D4 after depilation, dual-staining of Tomato and GFP, n = 3 mice per group. Scale bars, 50 μm