Fig. 4

Immunohistochemistry staining assay displayed PSD-95 and SYN positive cells of mouse brain tissue after scopolamine stimulation. The black arrow pointed to positive cells. The scale bar is labeled as 20 μm. The Representative images of immunohistochemistry staining in each group (A). The quantitative percentage of positive cells in total cells of PSD-95 and SYN was assessed in each group (B). Data are shown as mean ± SD, n = 3. #p < 0.05, ##p < 0.01 and ###p < 0.001 compared to the control group; *p < 0.05, **p < 0.01 and ***p < 0.001 compared to the scopolamine-treated group