Fig. 7

Effects of ASF on cell viability and H₂O₂-induced oxidative stress in PC12 cells. Cells were pre-treated with ASF (0.1, 1, 10ug/mL) for 24 h, and then stimulated with H₂O₂ (175uM) for 4 h. To assess the toxicity of ASF extracts and H₂O₂ to cells, we performed CCK8 assay in vitro (A). The ROS levels were detected by laser confocal microscopy (B). The levels of MDA (D) and activities of SOD, CAT, and GSH (C, E, F) in cells were determined by commercial assay kits. Data were expressed as mean ± SE (n = 3). ^#p < 0.05, ^##p < 0.01 and.^###p < 0.001 significantly different from control group; *p < 0.05, **p < 0.01 and ***p < 0.001 significantly different from the H₂O₂- stimulated group