Fig. 6

miR-21 recovery impaired the effects of DIM on MDSCs. A The percentage of total MDSCs and their subsets after BM-induced MDSCs (in the condition of 40 ng/ml GM-CSF and 40 ng/ml IL-6) were treated with DIM and miR-21 precursors for 4 days and examined by flow cytometry. B Relative miR-21 level and C Arg1/iNOS mRNA levels were examined in differently treated MDSCs. D The percentage of CD4⁺ and CD8⁺ T cells was measured by flow cytometry after BM-induced MDSCs were cocultured with DIM and pre-miR-21. Data are representative results from three independent experiments and the results are expressed as the mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001. T-MDSC: total MDSCs; M-MDSC: monocytic MDSC; G-MDSC: granulocytic MDSC