Fig. 5

Effects of daidzin and daidzein on MAPK signaling pathways. A TLR4, B phosphorylation of p38 at Thr180/Tyr182, C ERK at Thr202/Tyr204, and D JNK at Thr183/Tyr185 normalized to its corresponding total protein in RAW264.7 cells pretreated with daidzin (50 µM) for 4 h and co-treated with LPS (1 µM) for another 12 h. E–H Protein expression of TLR4, phosphor-p38, phosphor-ERK and phosphor-JNK for daidzein treatment (50 µM) with LPS. Values are the means ± SEM (n = 4); *p < 0.05, **p < 0.001, daidzin vs. LPS and daidzein vs. LPS; ^#p < 0.05, ^##p < 0.01, LPS vs. control