Fig. 2

A and B H1688 and A549 cells were treated with NC with or without Z-VAD-FMK, DSF, IM-54, Fer-1, Nec-1 respectively. After 48 h of treatment, the inhibition of growth was assayed. C H1688 and A549 cells were treated with NC (0.5 μm) or DDP (15 μm) for 48 h and microscopic imaging was captured. Arrowheads indicated ballooned cell membrane characteristic of pyroptotic cells. D H1688 and A549 cells were treated with 0.5 and 1 μm NC or 15 μm DDP for 48 h, hoechst 33,342 and PI dye were added and visualized under an inverted fluorescence microscope. Yellow arrowheads indicated the large bubbles emerging from the plasma membrane. E H1688 and A549 cells were treated with NC or DDP at indicated concentrations for 48 h, the release of LDH in cell supernatants were analyzed using LDH assay kit and expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs control