Fig. 5

The cells were pre-treated with 20 μm 740Y-P or 0.5 μm NC alone or combined with 20 μm 740Y-P for 48 h. WB analyses were performed to detected the protein levels of PI3K, p-PI3K, Akt, p-Akt, GSDME, cas 3, cl-cas 3, Bcl-2, Bax and β-Actin. Protein levels were expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs control