Fig. 4

The effects of XYT on hypoxia-induced RV and pulmonary vascular remodeling in rats. A Time-dependent body weight in different groups (n = 7). B RVHI: the ratio of right ventricular weight to left ventricular weight plus septum weight (RV/(LV + S) × 100%). C RVW/BW: the ratio of RV weight to body weight. D Representative micrographs stained with H&E staining in the RV. E Masson's trichrome staining (collagen fibers were stained blue) in the RV. Original magnification × 400, scale bar = 50 μm. F Quantitation of collagen content in the RV. G H&E staining in the pulmonary arteries. H WT%: the percentage of medial wall thickness. I WA%: the percentage of medial wall area. J Masson's trichrome staining in the pulmonary arteries. Original magnification× 400, scale bar = 50 μm. K Quantitation of lung perivascular collagen content. n = 6; ***p < 0.001 vs. control group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. hypoxia group. Statistical analyses were conducted using one-way analysis of variance (ANOVA)