Analyte | Nominal concentration (ng/mL) | Intra-day (n = 5) | Inter-day (n = 5) | ||||
---|---|---|---|---|---|---|---|
Measured concentration (mean ± SD, ng/mL) | Precision (RSD%) | Accuracy (RE%) | Measured concentration (mean ± SD, ng/mL) | Precision (RSD%) | Accuracy (RE%) | ||
Baicalin | 2 | 2.00 ± 0.22 | 11.23 | – 0.13 | 2.02 ± 0.20 | 9.71 | 0.78 |
4 | 4.04 ± 0.17 | 4.10 | 0.99 | 4.01 ± 0.30 | 7.57 | 0.23 | |
20 | 18.77 ± 0.96 | 5.11 | – 6.15 | 18.91 ± 1.21 | 6.42 | – 5.44 | |
200 | 211.08 ± 3.43 | 1.63 | 5.54 | 212.40 ± 7.49 | 3.53 | 6.20 | |
Ursodeoxycholic acid | 10 | 10.90 ± 0.54 | 4.93 | 8.96 | 10.68 ± 0.52 | 4.85 | 6.83 |
20 | 17.77 ± 0.38 | 2.13 | – 11.17 | 17.46 ± 0.51 | 2.93 | – 12.71 | |
100 | 87.02 ± 1.19 | 1.37 | – 12.98 | 86.00 ± 2.16 | 2.51 | – 14.00 | |
1000 | 1119.11 ± 26.08 | 2.33 | 11.91 | 1081.33 ± 53.16 | 4.92 | 8.13 | |
Chenodeoxycholic acid | 1 | 0.94 ± 0.04 | 4.10 | – 5.63 | 0.99 ± 0.08 | 8.30 | – 1.30 |
2 | 1.98 ± 0.05 | 2.39 | – 1.23 | 2.06 ± 0.10 | 4.70 | 3.00 | |
10 | 9.13 ± 0.36 | 3.98 | – 8.71 | 9.74 ± 0.94 | 9.61 | – 2.61 | |
100 | 94.07 ± 3.89 | 4.14 | – 5.93 | 97.31 ± 4.01 | 4.12 | – 2.69 | |
Chlorogenic acid | 1 | 0.97 ± 0.08 | 8.75 | – 3.17 | 1.01 ± 0.08 | 7.77 | 1.46 |
2 | 1.96 ± 0.15 | 7.77 | – 2.07 | 2.11 ± 0.16 | 7.71 | 5.27 | |
10 | 9.49 ± 0.73 | 7.70 | – 5.09 | 9.50 ± 0.91 | 9.54 | – 5.01 | |
100 | 97.86 ± 1.82 | 1.86 | – 2.14 | 100.09 ± 4.63 | 4.62 | 0.09 |