Analyte | Nominal concentration (ng/mL) | Short-term stability (6 h at room temperature) | Long-term stability (30 days at – 80 °C) | Freeze-thaw stability (3 cycles) | |||
---|---|---|---|---|---|---|---|
Mean ± S.D concentration (ng/mL) | RSD (%) | Mean ± S.D. concentration (ng/mL) | RSD (%) | Mean ± S.D. concentration (ng/mL) | RSD (%) | ||
Baicalin | 4 | 4.01 ± 0.31 | 7.75 | 3.50 ± 0.07 | 2.00 | 3.97 ± 0.36 | 9.12 |
20 | 18.43 ± 0.75 | 4.05 | 17.47 ± 0.42 | 2.42 | 17.88 ± 0.92 | 5.13 | |
200 | 182.83 ± 7.72 | 4.22 | 175.01 ± 4.57 | 2.61 | 202.81 ± 10.87 | 5.36 | |
Ursodeoxycholic acid | 20 | 19.38 ± 0.97 | 5.01 | 18.77 ± 0.41 | 2.20 | 18.47 ± 1.66 | 9.00 |
100 | 97.83 ± 0.62 | 0.63 | 90.80 ± 1.57 | 1.73 | 87.08 ± 1.95 | 2.24 | |
1000 | 1104.46 ± 16.75 | 1.52 | 1081.75 ± 20.70 | 1.91 | 1048.41 ± 75.10 | 7.16 | |
Chenodeoxycholic acid | 2 | 1.77 ± 0.06 | 3.21 | 1.92 ± 0.22 | 11.27 | 1.97 ± 0.17 | 8.89 |
10 | 9.79 ± 0.78 | 7.94 | 10.10 ± 0.36 | 3.54 | 9.37 ± 0.95 | 10.16 | |
100 | 101.57 ± 0.54 | 0.53 | 101.23 ± 5.13 | 5.07 | 95.70 ± 4.66 | 4.87 | |
Chlorogenic acid | 2 | 1.98 ± 0.16 | 8.27 | 1.73 ± 0.05 | 3.00 | 2.07 ± 0.17 | 8.16 |
10 | 8.97 ± 0.70 | 7.75 | 8.95 ± 0.42 | 4.65 | 10.03 ± 0.84 | 8.33 | |
100 | 88.28 ± 2.72 | 3.08 | 87.31 ± 4.62 | 5.30 | 95.65 ± 3.72 | 3.89 |