Fig. 3

TPLR inhibited the proliferation and secretion on HSCs. The CCK-8 assay of the proliferation on A L929, B LX-2 and C HSC-T6 cells with different concentrations of TPLR (0, 10, 25, 50, 100 and 200 μg/ml) incubated for 48 h (n = 4). D The images of EdU staining on LX-2 cells with TPLR (0, 50, 100 and 200 μg/ml) for 48 h. In the representative EdU images, total cells stained with DAPI were blue, whereas proliferating cells were shown as red point, and the ratio of red in blue cells (n = 3), scale bars: 100 μm. E Quantitative analysis of EdU staining of proliferation on the LX-2 cells with different concentrations of TPLR treatment (n = 3) (F–G) The mRNA level of FN, Col1α1 and α-SMA in LX-2 cells with 100 μg/ml TPLR for 48 h. Data were shown as mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 ^****P < 0.0001, vs. control group