Fig. 4

TPLR significantly induced the apoptosis on LX-2 cells. A The apoptosis effect of LX-2 cells with 0, 50, 100 or 200 μg/ml TPLR for 48 h by flow cytometry. B Quantitative analysis of A. C The levels of caspase-3, -8, -9, -10 after an incubation with various concentrations of TPLR (0, 50, 100 or 200 μg/ml) for 48 h by Western blot. D Quantitative analysis for western blotting (n = 3). The results are shown as mean ± SD; *P < 0.05, **P < 0.01, compared with the control group. E The apoptosis rates of LX-2 cells with TPLR (0, 100 or 200 μg/ml) for 12 h in the presence of TGF-β1 (10 ng/ml) for 12 h by flow cytometry. F Quantitative analysis of E. G The western blot results of the caspase family in LX-2 cells with TPLR (0, 50, 100 or 200 μg/ml) for 12 h with 10 ng/ml TGF-β1. H Quantitative analysis for western blotting (n = 3). The results are shown as mean ± SD; ^#P < 0.05, compared with the control group; *P < 0.05, **P < 0.01, ***P < 0.001 compared with the TGF-β1 group