Fig. 7

AMPK activation is required for honokiol-mediated beneficial effects in vivo. A Schematic of the experimental procedure used with mice fed a CDAHFD diet and treated with vehicle or honokiol (100 mg/kg) in the absence or presence of compound C (CC, 10 mg/kg, every other day, i.p.). B Western blots of p-AMPKα, AMPKα, p-ACC, and ACC of mice in the indicated groups. n = 3 mice per group. C The blood glucose concentration during GTT of CDAHFD-fed mice in the indicated groups. n = 6 mice per group. One-way ANOVA was applied for statistical analysis. P-values of the red color represent the comparison of PBS-vehicle vs PBS-honokiol, while black represents CC-vehicle vs CC-honokiol. D Liver contents of TG, TC, and NEFA of CDAHFD-fed mice in the indicated groups. n = 6 mice per group. The Kruskal–Wallis test was applied for statistical analysis. E Representative images of the indicated mouse liver sections stained with HE, ORO, PSR, and IHC for CD11b-positive cells. n = 6 mice per group. Scale bar, 50 μm. F Results of NAS (HE) and quantitative analysis of ORO, PSR, and CD11b data shown in (E). n = 6 mice per group. For statistical analysis, the Kruskal–Wallis test was used for NAS and one-way ANOVA was applied to ORO, PSR, and CD11b data. G Serum ALT and AST activity of CDAHFD-fed mice shown in the indicated groups. n = 6 mice per group. Student’s t-test was applied for statistical analysis. H Serum TC concentrations in CDAHFD-fed mice treated with the indicated groups. n = 6 mice per group. Student’s t-test was applied for statistical analysis. I Dot plot representing pairwise GSVA comparisons of transcriptomic data from CDAHFD-fed mice shown in the indicated groups. J Heatmap of transcriptomic data from CDAHFD-fed mice shown in the indicated groups