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Fig. 5 | Chinese Medicine

Fig. 5

From: Suxiao Jiuxin Pill alleviates myocardial ischemia–reperfusion injury through the ALKBH5/GSK3β/mTOR pathway

Fig. 5

SJP inhibits autophagic activity through the ALKBH5 and GSK3β/mTOR pathway in gene knockout experiments following H/R (n = 3). a and b H9c2 cardiomyocytes underwent transfection with adenovirus carrying tandem fluorescent mRFP-GFP-LC3 (Ad-LC3-H9c2s) for 24 h and various treatments (bar = 50 μm). c and d The protein amounts of p-GSK3β/GSK3β, p-mTOR/mTOR, LC3B (II/I), Beclin-1, and p62 in H/R-treated H9c2 cells by western blot. e and f Apoptotic rates of cardiomyocytes in H/R-induced H9c2 cells assessed by the Annexin V/PI assay. Data are mean ± SEM. *P < 0.05 versus shRNA-Ctrl + H/R group; # P < 0.05 versus shRNA-ALKBH5 + H/R group; &P < 0.05 versus shRNA-GSK3β + H/R group

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