Fig. 6

SJP inhibits excessive autophagy through the ALKBH5/GSK3β pathway in H/R-induced H9c2 cells (n = 3). a and b H9c2 cardiomyocytes were transfected with adenoviruses harboring tandem fluorescent mRFP-GFP-LC3 (Ad-LC3-H9c2s) for 24 h and subjected to various treatments (bar = 50 μm). c and d Numbers of autophagosomes determined by TEM (× 40,000). e and f Apoptotic rates of H/R-treated H9c2 cells assessed by the Annexin V/PI assay. g and h Protein levels of p-GSK3β/GSK3β, p-mTOR/mTOR, LC3B (II/I), Atg5, and p62 in H/R-treated H9c2 cells evaluated by western blot. Data are mean ± SEM. ^*P < 0.05 versus shRNA-ALKBH5 + H/R group; ^#P < 0.05 versus shRNA-ALKBH5 + GSK3β-inhibitor + H/R group