Fig. 1

FZHY effectively reverses CCL4-induced liver fibrosis in mice. A Six-week-old mice were injected intraperitoneally with 10% CCL4 at a dose of 2 ml/kg, three times a week for 6 weeks. After 2 weeks of CCL4 injection, daily treatment with FZHY at 5.6 g/kg began. The normal control group received vehicle as control. Six weeks later, the mice were sacrificed 48 h after the last CCL4 injection. B Serum levels of serum ALT and AST were assayed by using commercial kits. C Representative images of H&E staining, Sirius red staining and immunohistochemical staining (H&E staining, magnification 200x, Sirius red and immunohistochemical staining, magnification 100x). D Semiquantification data were obtained by analysing Sirius red staining and immunohistochemical staining liver sections. Data were acquired using Image-Pro Plus software. E Liver fibrosis staging data assessed by Sirius red staining which using Redit analysis. F Hyp contents were quantified from 100 mg liver samples and were measured by Jamall’s method. Values represent the means ± SDs