Fig. 4

The influence of retinol metabolism pathway mainly comes from HSCs in vivo. A The Cyp4a12a/b enzyme of liver tissue were stained with Pinpo RNA in situ hybridization. B The immunofluorescence staining of α-SMA (red immunofluorescence) and RAE-1 (green immunofluorescence). C Primary HSCs and hepatocytes were isolated from mice with CCL4-induced liver fibrosis were identified by RT‒PCR for Desmin, Alb, F4/80, Vwf. D The relative mRNA expression levels for Cyp4a12a/b were determined from HSCs and hepatocytes. E The relative mRNA expression levels for RAE-1 were determined from HSCs. Values represent the means ± SDs